CMV recombinant antigens

Cytomegalovirus (CMV) or human herpes virus type 5 (HHV5) is a DNA-containing representative of the herpesvirus group. CMV is a member of the TORCH group of infections, so identifying CMV infection in pregnant women is critical due to the potential for harm to the fetus.
Only a positive CMV IgG test cannot clearly indicate the time that has passed since infection. At the same time, it is possible to distinguish primary infection from long-term persistence of the virus by determining the avidity of class G antibodies. After the first infection, IgG binding is initially weak and then increases, that is, avidity increases within 2-4 months. The infection is often asymptomatic due to variability of individual response and virulence of CMV strains.
To monitor the serological response, recognized markers include proteins P150(UL32), p52(UL44), as well as P28(UL99) and p65.
UL32(p150) is vital for CMV replication, capsid assembly, and virus exit from the cell.
UL99 increases the expression of viral genes, especially early genes that needed to initiate viral replication.
UL99(p28) interacts with host cell proteins to influence transcription, signalling, and apoptosis.
UL44(p52), part of the viral DNA polymerase complex, is critical for viral genome replication. It interacts with viral and host proteins to modulate gene expression and assist the assembly of viral particles.
pUL65 (p65), a tegument protein, assists the assembly and release of CMV virions. It interacts with viral proteins, strengthening the integrity of the virion.

We offer recombinant antigens produced by Vitrotest that imitate the main protein fragments, such as p150, p28, p52, p65. Using a mixture of these recombinant and native proteins allows ELISA to achieve high sensitivity. Recombinant CMV proteins have been tested for suitability in ELISA by many years of experience.