| Product description | The recombinant protein is an analogue of Major Outer Membrane protein (MOMP) of Chlamydia trachomatis, expressed and derived from E.coli |
| Purification methods | Purified by affinity chromatography |
| Buffer composition | 50mM Tris, 300mM NaCl, 8M Urea, 250 mM imidazole, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
Chlamydia trachomatis (C. trachomatis) is a Gram-negative bacterium that causes a variety of human infections known as chlamydia. This intracellular parasite has a unique development cycle, which includes two forms of existence: metabolically active non-infectious intracellular reticular bodies and metabolically inactive extracellular infectious elementary bodies. Chlamydia can exit from the host cell without lysis causing moderate infection.
Diagnosis primarily involves genetic tests, and serological tests detect persistent antibodies even after the infection has resolved. IgA antibodies appear in the blood 10-14 days after the onset of the disease; high concentrations of antibodies of this class may indicate a chronic infection. Specific IgA has a half-life of 5-7 days, allowing to monitor the effectiveness of treatment. IgG antibodies indicate acute, chronic or past infection and appear starting from the third week after the onset of the disease.
Crucial to serological assays is the major outer membrane protein (MOMP) of C. trachomatis. The three-dimensional structure of MOMP reveals variable domains recognized by the host immune system.
Another important antigen, heat shock protein 60 (HSP60), functions as a chaperone, assisting protein folding and triggering immune responses. Both proteins are highly immunogenic.
Vitrotest offers a recombinant protein similar to MOMP, produced with an E. coli expression system.