Hepatitis C virus is a human-pathogenic small single-stranded RNA virus of the Flaviviridae family. HCV causes hepatitis C, hepatocellular carcinoma and lymphoma.
Detection of circulating antibodies against HCV using serological methods has been the main approach to diagnosing infection. The immunodominant antigens HCcore, E2, NS3, NS4A/B and NS5A are used in the 3rd generation test kits. The humoral response to these antigens depends on the stage of the disease and the genotype of the virus. Today, there are 8 HCV genotypes, which are designated by numbers from 1 to 8, and almost 100 subtypes, which have a letter designation. Genotypic differences manifest themselves in changes in the antigenic characteristics of the virus, which should be taken into account when creating diagnostic kits to achieve the desired test sensitivity.
There are structural: HCcore, E1/E2 and non-structural (NS) HCV proteins. Envelope glycoproteins E1 and E2 are responsible for recognizing cell receptors. HCcore forms the viral capsid and promotes binding to the host lipid membrane. This protein is actively released into the bloodstream during viral replication and is an early antigenic marker. In most infected people, antibodies to NS3 and HCcore appear first, and only 20-22 weeks after infection antibodies to the non-structural protein NS4 are produced.
NS3 is a bifunctional enzyme with serine protease and RNA helicase domains. NS4 is responsible for attachment to the endoplasmic reticulum and mitochondria and serves as a cofactor for NS3. The NS5 complex is an RNA-dependent RNA polymerase (RdRp) and involved in the formation of double membrane vesicles (DMVs).
Produced by Vitrotest recombinant proteins are derived from E. coli and contain epitopes of the immunogenic proteins HCV HCcore, NS3, NS4 and NS5. These antigens are type-specific and were characterized on a large sample of sera from patients with hepatitis C, and also underwent thorough and many years of testing in ELISA kits. Our specialists will be happy to advise and recommend the best list of antigens for your production.
HCV recombinant antigen CNgst
| Product description | The mosaic recombinant protein contains immunodominant regions of core, NS3, NS4 antigens of Hepatitis C virus |
| Purification methods | Extraction of recombinant protein from washed Inclusion Bodies |
| Buffer composition | 0.02M Tris, 0.01M EDTA, 6MUrea, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen HCcore
| Product description | The recombinant protein contains the HCV core immunodominant regions, expressed and derived from E.coli |
| Purification methods | Purified by affinity chromatography |
| Buffer composition | 20mM Tris-HCl, 500mM NaCl, 8M Urea, 250mM imidazole, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 11
| Product description | The recombinant protein contains HCV NS3 immunodominant regions, expressed and derived from E. coli in soluble form |
| Purification methods | Purified by affinity chromatography |
| Buffer composition | 50mM Tris, 5mM Glutathione, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 13
| Product description | The recombinant protein contains HCV NS3 immunodominant regions, expressed and derived from E. coli |
| Purification methods | Purified by affinity chromatography |
| Buffer composition | 50mM Tris, 5mM Glutathione, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 1a
| Product description | The recombinant protein contains HCV genotype-1a NS3 immunodominant regions, expressed and derived from E.coli, affinity purified |
| Purification methods | Purified by affinity chromatography |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 1b
| Product description | The recombinant protein contains HCV genotype-1b NS3 immunodominant regions, expressed and derived from E.coli, affinity purified |
| Purification methods | Purified by affinity chromatography |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 2b
| Product description | The recombinant protein contains HCV genotype-2b NS3 immunodominant regions, expressed and derived from E.coli, affinity purified |
| Purification methods | Purified by affinity chromatography |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 3a
| Product description | The recombinant protein contains HCV genotype-3a NS3 immunodominant regions, expressed and derived from E.coli, affinity purified |
| Purification methods | Purified by affinity chromatography |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS3 6a
| Product description | The recombinant protein contains HCV genotype-6a NS3 immunodominant regions, expressed and derived from E.coli, affinity purified |
| Purification methods | Purified by affinity chromatography |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS4
| Product description | The recombinant protein contains HCV NS4 immunodominant regions, expressed and derived from E.coli in soluble form |
| Purification methods | Purified by affinity chromatography |
| Buffer composition | 50mM Tris-HCl, 5mM Glutathione, 150mM NaCl, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |
HCV recombinant antigen NS5
| Product description | The recombinant protein contains HCV NS5 immunodominant regions, expressed and derived from E.coli |
| Purification methods | Purified by affinity chromatography |
| Buffer composition | 50mM Tris, 5mM Glutathione, pH8.0 |
| Preservative | None |
| Storage | Frozen at -70 ˚C, avoid repeated freeze-thaw cycles |