Opisthorchis felineus indirect ELISA controls

Enzyme-linked immunosorbent assay (ELISA) is a key diagnostic tool for opisthorchiasis. In this diagnostic procedure, a positive control, usually in the form of serum, is used to confirm that the laboratory test was performed correctly. Sera containing specific antibodies to Opisthorchis felineus are quite rare, which poses a challenge for mass production. In addition, this material may pose a risk of transmission of infection.
To avoid these problems, we propose synthetic positive controls based on monoclonal antibodies. These synthetic controls generate various advantages, including increased stability, reduced batch-to-batch variation, availability, and ease of standardization. For many years, these synthetic controls have demonstrated good results in the production of diagnostic kits.
In addition, the use of stabilizers developed by us ensures long-term preservation of the activity of internal controls.